ABSTRACT
Objective To investigate the bacterial resistance profile of clinical isolates collected in the hospitals across Chuzhou in 2016. Methods Antimicrobial susceptibility testing was carried out by Kirby-Bauer method. The data were analyzed using WHONET 5.6 software according to CLSI 2015 breakpoints. Results A total of 5 465 clinical isolates were collected during 2016, of which gram positive organisms and gram negative organisms accounted for 25.9％ (1 416/5 465) and 74.1％ (4 049/5 465), respectively. Prevalence of MRSA was 37.6％ among S. aureus and the prevalence of MRCNS was 78.1％ in CNS. All Staphylococcus, E. faecalis and E. faecium isolates were sensitive to vancomycin and linezolid. The prevalence of extended spectrum-lactamases (ESBLs) positive strains was 51.2％ in E. coli, 23.4％ in Klebsiella spp. (K. pneumoniae and K. oxytoca), and 23.6％ in P. mirabilis isolates, respectively. The Enterobacteriaceae strains were highly sensitive to carbapenems. The percentage of the P. aeruginosa isolates resistant to the antimicrobials tested was lower than 30％. The percentage of the Acinetobacter strains resistant to meropenem and imipenem was 65.6％ and 67.4％, respectively. Conclusions The situation of antibiotic resistance is still very serious, especially multi-drug or pan-drug resistant strains, which is of great concern.
ABSTRACT
Objective To investigate the distribution and antibiotic resistance of bacterial isolates from Children's Hospital of Chuzhou during the period from January 2014 through December 2016.Methods A retrospective analysis was carried out for 1 057 bacterial strains isolated from clinical specimens from January 2014 through December 2016.Results Of the 1 057 clinical isolates collected,gram positive organisms and gram-negative organisms accounted for 45.3％ (479/1 057) and 54.7％ (578/1 057),respectively.The top 5 most frequently isolated microorganisms were E.coli (19.6％),S.aureus (15.9％),Klebsiella spp.(14.4％),coagulase negative Staphylococcus (12.6％) and S.pneumoniae (9.1％).All the S.pneumoniae isolates were susceptible to penicillin.Prevalence of MRSA was 29.8％ among S.aureus and the prevalence of MRCNS was 74.4％ in CNS.All Staphylococcus strains were susceptible to daptomycin,linezolid and vancomycin.E.faecium strains showed much higher resistance rate to most of the antimicrobial agents tested than E.faecalis.The prevalence of ESBLs-positive strains was 50.2％ in E.coli,32.4％ in Klebsiella spp.(K.pneumoniae and K.oxytoca) and 83.3％ in P.mirabilis,respectively.The Enterobacteriaceae strains were highly sensitive to carbapenems.A total of 12 (2.5％) carbapenem-resistant strains were identified from 485 Enterobacteriaceae isolates.P aeruginosa and Acinetobacter spp.were highly sensitive to carbapenems.Conclusion Gram negative bacteria are still dominant pathogens in clinical practice.It is necessary to strengthen monitoring of antibiotic resistance,which is still a serious issue of great concern.
ABSTRACT
Objective To investigate the distribution and antimicrobial resistance profile of the bacterial strains isolated from Chuzhou First People's Hospital in 2016.Methods The bacterial isolates were identified and tested for antibiotic susceptibility using MicroScan WalkAway 96 PLUS bacterial identification system.All the data were analyzed with WHONET 5.6 software.Results A total of 1 441 clinical isolates were collected during 2016,of which gram-positive organisms accounted for 28.4％ and gram-negative organisms 71.6％.Prevalence of methicillin-resistant strains in S.aureus (MRSA) and coagulase negative Staphylococcus (MRCNS) was 37.5％ and 83.0％,respectively.All Staphylococcus isolates were susceptible to vancomycin,daptomycin and linezolid.All E.faecium isolates were susceptible to daptomycin,vancornycin and linezolid.One strain (2.6％) of E.faecalis was resistant to linezolid.All Streptococcus pneumoniae strains (mainly isolated from children) were susceptible to penicillin.The prevalence of extended-spectrum beta-lactamases (ESBLs) positive strains was 49.1％,24.2％ and 62.5％ in E.coli,Klebsiella (K.pneumoniae and K.oxytoca) and P mirabilis,respectively.The resistance rate of ESBLs-positive strains was higher than ESBLs-negative strains to most antibiotics.The percentage of the P.aeruginosa isolates resistant to meropenem and imipenem was all 10.4％.The percentage of Acinetobacter (A.baumannii accounted for 95.0％) strains resistant to imipenem and meropenem was 79.0％ and 78.2％.Conclusions The situation of antibiotic resistance is still very serious in 2016 in this hospital.The prevalence of carbapenemresistant K.pneumoniae and 4.baumannii is still increasing,which is of great concern.